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KMID : 0370220220660060331
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Yakhak Hoeji
2022 Volume.66 No. 6 p.331 ~ p.335
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Effect of Diet Restriction on Renal Glutathione Metabolism in Mice
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Lee So-Min
Bae Ji-Eun
Kwon Do-Young
Jung Young-Suk
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Abstract
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Various benefits for the maintenance of metabolic homeostasis can be obtained through dietary restriction.
Short-term fasting of mice may induce systemic metabolic remodeling, which may have a significant effect on the levelof endogenous metabolites. This study was conducted to examine metabolic changes of sulfur-containing substances in thekidneys of fasted mice over 48 h. No change in the concentration of renal methionine was observed over 48 h; however,significant decreases in the concentrations of S-adenosylmethionine and S-adenosylhomocysteine were observed at both 24and 48 h after fasting. A significant decrease in the concentration of renal cysteine was observed from 24 h, and this wasmaintained at 48 h. A significant decrease in the concentration of glutathione (GSH) was observed in the kidney at 48 h,whereas increased concentrations of taurine were observed 48 h after fasting. Although significantly increased expressionlevels of BHMT were observed, markedly decreased expression levels of CβS were observed at 48 h after fasting,indicating activated generation of methionine, but downregulation of cysteine synthesis. The expression level of γ-GCL wasnot altered by fasting for 48 h; however, a significant increase of cysteine sulfinate decarboxylase mediating the taurinesynthesis was observed. Significantly decreased protein expression levels of solute carrier family 7 member 11, whichimports extracellular cystine, were observed at 48 h. These results suggest that the renal methionine concentration wasmaintained during short-term fasting through an increase in the re-methylation of homocysteine. Moreover, a reduction inthe concentration of cysteine was the result of an increase in the synthesis of taurine and a decrease in the uptake ofextracellular cystine, which may result in a decreased concentration of renal GSH.
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KEYWORD
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Diet restriction, Kidney, Methionine, Glutathione
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